Journal of Applied Dental
and Medical Sciences
Journal of Applied Dental and Medical Sciences is A Quarterly Published

ABSTRACT

To compare the influence of different irrigation solutions on calcium ion loss and microhardness of root dentin- An in vitro study

Sadashiv Daokar, Ankit Sable, Kalpana Pawar, Snehal Tawar, Ajay Jadhav, Neha Chavan

ABSTRACT

Aim: To compare and evaluate the calcium ion loss and microhardness of root dentin using sodium hypochlorite, peracetic acid, and chitosan and apple cider vinegar. Methods: GI-sodium hypochlorite 5%,GII- peracetic acid 1%, GIII- chitosan 2% and GIV-apple cider vinegar. Fourty single rooted freshly extracted premolars were decoronated at the cemento-enamel junction and at apical end of the root using a diamond disc to obtain 10mm length which was standardized for all the specimens in different groups. The pulp extirpation and root canal enlarged using K files up to ISO # 25. Then sectioned longitudinally into two halves making a total of 40 samples and other half was discarded. Then using magnetic stirrer test solutions were prepared for each group and then spectrometric analysis and microhardness test were done. Data collected was analyzed using SPSS software. Level of significance was fixed at p= 0.05 Result: Mean calcium ion loss was highest for Group III followed by Group IV, Group II and Group I. Mean microhardness was lowest for Group III followed by Group IV, Group II and Group I. Group III had the highest amount of calcium ion loss and lowest microhardness, which was significantly different from Group I, II and IV. Conclusion: Chitosan 2% resulted in maximum sequestration of calcium ions present amongst the chelating solutions, followed by apple cider vinegar, peracetic acid 1% and sodium hypochlorite. The highest microhardness of root dentin was seen with sodium hypochlorite and peracetic acid.

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